• Active Recombinant Mouse IL-4 Protein 重组蛋白 动物活性

    Active Recombinant Mouse IL-4 Protein 重组蛋白 动物活性

  • 2023-04-04 18:34 50
  • 产品价格:23.00
  • 发货地址:湖北省武汉市包装说明:不限
  • 产品数量:不限产品规格:不限
  • 信息编号:102845273公司编号:4277473
  • 陈尧 销售总监
    +8617508640520 (联系我请说明是在阿德采购网看到的信息)
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    产品描述
    描述Active Recombinant Mouse IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (His23-Ser140) of mouse IL-4 (Accession #NP_067258.1) fused with a 6×His tag at the C-terminus.
    生物活性asured by its ability to induce Mouse Bone marrow derived macrophage (BMDM) differentiation into M2-type. 20ng/mL of Recombinant Mouse IL-4 and Mouse IL-13 (RP01123) can effectively upregulate the expression of M2-type biomarker Arg-1.
    2.10 ng/mL of Mouse IL-4 and 20 ng/mL of Mouse GM-CSF induce C57BL/6J Mouse cells differentiate into BMDC. According to the Flow cytometry data, after 6 days, up to 75% of cells in the system expressed DC biomarker CD11C on cell surface, among which more than 15% cells have high expression of MHCII. These data shows that the induction was successful.
    3.Recombinant Mouse IL-4 protein (10 ng/mL) and Mouse GM-CSF protein (20 ng/mL) induce C57BL/6 Mouse bone marrow cells to differentiate into BMDC. After 7 days, up to 89.4% of cells in the system expressed DC biomarker CD11c on cell surface, among which more than half of the cells have high expression of CD80 and CD86 proteins. These data shows that the induction was successful.
    asured in a cell proliferation assay using MC/9 2 mouse mast cells. The ED50 for this effect is 2.8-11.3 pg/mL.

    靶点背景信息

    基因ID16189
    Swiss ProtP07750
    别名Il-4; BSF-1;IL4







    武汉瑾萱生物科技有限公司是一家专注于重组蛋白,抗体早期发现,细胞培养的CRO服务的生物科技企业。
       用认真严谨的技术水平,积极的响应态度对待每一位行业用户。

    欢迎来到武汉瑾萱生物科技有限公司网站,我公司位于江河纵横、湖港交织,交通发达,文化发达的武汉市。 具体地址是湖北武汉公司街道地址,负责人是陈尧。
    主要经营重组蛋白、抗体制备、细胞培养、elisa试剂盒。
    单位注册资金:人民币 10 万元 - 30 万元。
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武汉瑾萱生物科技有限公司是一家专注于重组蛋白,抗体早期发现,细胞培养的CRO服务的生物科技企业。 用认真严谨的技术水平,积极的响应态度对待每一位行业用户。
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